RandomConicalTilt

Random Conical Tilt

• Alignment of the untilted images.
• Transfer the in-plane rotation angles of the untilted images to their corresponding tilted pairs, and center the tilted images.
• Perform a 3D reconstruction with the tilted images.

Example Data

• Input
  • Gallery of tilted and untilted images. Previous alignment made with maximum likelihood is assumed.

File random_conical_tilt.tar.gz contains all the images and the xmipp_protocol_rct.py script necessary to run this protocol. This protocol needs a previous ML2D classification. For this example the local copy of the images have been made in order to reduce the size of the tar file.

• Output
  • gallery of aligned tilted images
  • 3D reconstruction

File output_random_conical_tilt.tar.gz contains the results of executing theRandon Conical Tilt script.

Gallery of untilted aligned images (top-right), slices of the reconstructed 3D map (bottom-left) and average of the untilted images (bottom-right)

Press`analyze results` button for visualizing the rotational spectra classification. ## USER's COMMENTS