DetectMissingWedge

Detect missing wedge

Purpose

This program allows you to detect the orientation of the missing wedge in a tomogram. For doing so it fits a couple of planes along which there is a maximum variation between the energy of the Fourier transform on its left and on its right. The missing wedge is coded with four angles (two for each plane). You may also produce a mask with 1 where the missing wedge is, and 0 where the data has been actually measured. Finally, you can also produce a marked magnitude volume (i.e., the magnitude of the Fourier transform where the position of the two planes have been marked).

Usage

$ detect_missing_wedge ...

Parameter

• `` Input tomogram. The output mask and mark volumes are called ..._mask.vol and ..._marks.vol
• -maxFreq [f0.25] = Maximum frequency (<0.5) to measure the differences between planes
• -width [w2] = How many pixels on each side of the plane should be considered for the comparison
• `` Save the marks volume (..._marks.vol)
• `` Save the mask volume (..._mask.vol)

Examples and notes

The most common call is

$ detect_missing_wedge -i tomogram.vol

--Main.CoSS - 07 Oct 2009