Quick Start

Quick Start

This page is the fastest practical path through a standard Icescopy session.

Before you start

Have these ready:

• an ordered image sequence
• a writable location for the session file
• optional temperature data files if you plan to import them later

Standard workflow

1. Open Icescopy.
2. Start a new session.
3. Add images or add an image folder.
4. Check the image order in the Images dock.
5. Annotate cells.
6. Assign samples if needed.
7. Run analysis.
8. Review the Grayscale Plot and the results tables.
9. Save the session.
10. Export results if needed.

Load images

Use:

• File -> Add Images...
• File -> Add Folder...

After loading, confirm:

• the image list is populated
• the frame slider covers the full sequence
• the first and last few images are in the expected order

Annotate cells

Use either:

• single-cell placement
• grid placement

If cells drift during the run:

• add keyframes at frames where the layout visibly changes
• adjust the cell positions there

Run analysis

Use the analysis command from the app UI after annotation is complete.

The app will populate:

• grayscale measurements
• freeze events
• freeze count timeseries results if external temperature data was imported

Save early

Save the session near the start of work instead of waiting until the end.

This helps with:

• relinking images later
• resuming work after interruption
• sharing the session file with the image folder structure intact

If something looks wrong

Check these first:

• image order
• cell placement
• whether a crop or image edit is active
• whether the results are stale and need a rerun