LaserCoral

Data and methods for Chapter Three of my dissertation, Laser Capture Microdissection RNAseq analysis

Repository organization

Sample metadata

• Metadata for all samples

RNA-seq Data QC and Processing

• Raw data: Uploaded to SRA, BioProject PRJNA1209584
• Bioinformatic pipeline
• QC of Raw and Trimmed reads
• Genome mapping rate per sample
• Gene count matrix

DeSeq2 differential expression

• DeSeq2 Differential Expression Analysis script
• GO Enrichment Analysis
• Biomineralization + Marker Gene Analysis (and all final figures)
• Outputs
  • GO Enrichment Outputs
  • Key outputs: paper supplementary tables

Biomineralization-related genes (Scucchia et al., 2021; Scucchia et al., 2021)

• Excel file
• Fasta file
• P. acuta orthologs

Stylophora pistillata scRNA-seq Marker Genes (Levy et al., 2021)

• Marker Gene File downloaded from here
• P. acuta orthologs

Pocillopora acuta species identification:

• FASTA and genious files of Pocillopora species trees

Reference Genome and annotations:

• Version 2 from: http://cyanophora.rutgers.edu/Pocillopora_acuta/

NCBI Sequence uploads:

• Bioproject: PRJNA1209584