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Meningococci

Processes

How sequential DNA data is handled

1. Sending is entered to the database and gets a new stemnumber
2. Laboratory assistant isolate DNA and marks it with stemnumber
3. A batch of isolated DNA is sent to sequencing (~every three month)
4. When sequencing is finished DNA sequence files are available
5. Sequence files are manually assembled fo Fastq-files. Each file is then named xxx.fas, where xxx=stemnumber.
6. It is possible to batch upload a list of Fastq-files to the database
7. Each Fastq-file name is verified to match a corresponding isolate by stem number
8. Corresponding database properties for PubMLST submission are extracted from the database (required fields?)
9. Meta properties and sequences are submitted to PubMLST and if successful the PubMLST isolate id is stored to the isolate record