microDBiT is a microfluidic-based spatial transcriptomics platform that enables simultaneous, high-resolution profiling of host and microbial RNAs—including both mRNA and rRNA—within the same tissue section.
Unlike conventional spatial transcriptomics methods that focus exclusively on eukaryotic transcripts, microDBiT integrates:
- Optimized microfluidic barcoding chemistry
- Tissue handling protocols designed to preserve microbial RNAs
- Compatibility with small and low-abundance bacterial populations
This technology opens new avenues for studying host-microbiome interactions at spatial resolution across complex tissues.
- Spatial mapping of both host and bacterial gene expression
- Retention and detection of microbial RNA (including rRNA)
- Compatible with complex and low-biomass microbiota
- Designed for tissue sections including gut, bladder, and more
python/— Scripts and notebooks used in the original data analysis pipelineimages/— Figures and diagrams used in publications or slides
For questions or collaborations, please contact Yang Liu, Ph.D. (Assistant Professor, Yale School of Medicine)