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/**
* NEB Restriction Enzymes
*
* a list of enzyme objects with their name as the key,
* their recognition site sequence as "rseq", and cut site relative to the
* start of the recognition site as "fcut", and the start of the resulting overhang
* from the recognition site as "rcut"
*
* eg: PstI with recognition site "CTGCAG" cuts so that the break is
* at (cutSite = 5):
* ..C TGCA|G..
* ..G|ACGT C..
*
* and the resulting fragment looks like (rcut = 1):
* ..CTGCA
* ..G****
*
* @typedef {Object} EnzymeInfo
* @property {String} rseq the recognition sequence associated with the enzyme
* @property {Number} rcut the index of the hangSite, relative to the rseq start
* @property {Number} fcut the cut index of the enzyme relative to rseq start
*/
export default {
"PI-SceI": {
rseq: "ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG",
rcut: 11,
fcut: 15
},
"PI-PspI": {
rseq: "TGGCAAACAGCTATTATGGGTATTATGGGT",
rcut: 13,
fcut: 17
},
"I-CeuI": {
rseq: "TAACTATAACGGTCCTAAGGTAGCGAA",
rcut: 14,
fcut: 18
},
"I-SceI": {
rseq: "TAGGGATAACAGGGTAAT",
rcut: 5,
fcut: 9
},
AscI: {
rseq: "GGCGCGCC",
rcut: 6,
fcut: 2
},
AsiSI: {
rseq: "GCGATCGC",
rcut: 3,
fcut: 5
},
FseI: {
rseq: "GGCCGGCC",
rcut: 2,
fcut: 6
},
NotI: {
rseq: "GCGGCCGC",
rcut: 6,
fcut: 2
},
PacI: {
rseq: "TTAATTAA",
rcut: 3,
fcut: 5
},
PmeI: {
rseq: "GTTTAAAC",
rcut: 4,
fcut: 4
},
PspXI: {
rseq: "VCTCGAGB",
rcut: 6,
fcut: 2
},
SbfI: {
rseq: "CCTGCAGG",
rcut: 2,
fcut: 6
},
SfiI: {
rseq: "GGCCNNNNNGGCC",
rcut: 5,
fcut: 8
},
SgrAI: {
rseq: "CRCCGGYG",
rcut: 6,
fcut: 2
},
SrfI: {
rseq: "GCCCGGGC",
rcut: 4,
fcut: 4
},
SwaI: {
rseq: "ATTTAAAT",
rcut: 4,
fcut: 4
},
BaeI: {
rseq: "NNNNNNNNNNNNNNNACNNNNGTAYCNNNNNNNNNNNN",
rcut: 33,
fcut: 38
},
BbvCI: {
rseq: "CCTCAGC",
rcut: 5,
fcut: 2
},
BspQI: {
rseq: "GCTCTTCNNNN",
rcut: 11,
fcut: 8
},
CspCI: {
rseq: "NNNNNNNNNNNNNCAANNNNNGTGGNNNNNNNNNNNN",
rcut: 35,
fcut: 37
},
PpuMI: {
rseq: "RGGWCCY",
rcut: 5,
fcut: 2
},
RsrII: {
rseq: "CGGWCCG",
rcut: 5,
fcut: 2
},
SapI: {
rseq: "GCTCTTCNNNN",
rcut: 11,
fcut: 8
},
SexAI: {
rseq: "ACCWGGT",
rcut: 6,
fcut: 1
},
AatII: {
rseq: "GACGTC",
rcut: 1,
fcut: 5
},
Acc65I: {
rseq: "GGTACC",
rcut: 5,
fcut: 1
},
AccI: {
rseq: "GTMKAC",
rcut: 4,
fcut: 2
},
AclI: {
rseq: "AACGTT",
rcut: 4,
fcut: 2
},
AcuI: {
rseq: "CTGAAGNNNNNNNNNNNNNNNN",
rcut: 20,
fcut: 22
},
AfeI: {
rseq: "AGCGCT",
rcut: 3,
fcut: 3
},
AflII: {
rseq: "CTTAAG",
rcut: 5,
fcut: 1
},
AflIII: {
rseq: "ACRYGT",
rcut: 5,
fcut: 1
},
AgeI: {
rseq: "ACCGGT",
rcut: 5,
fcut: 1
},
AhdI: {
rseq: "GACNNNNNGTC",
rcut: 5,
fcut: 6
},
AleI: {
rseq: "CACNNNNGTG",
rcut: 5,
fcut: 5
},
AlwNI: {
rseq: "CAGNNNCTG",
rcut: 3,
fcut: 6
},
ApaI: {
rseq: "GGGCCC",
rcut: 1,
fcut: 5
},
ApaLI: {
rseq: "GTGCAC",
rcut: 5,
fcut: 1
},
ApoI: {
rseq: "RAATTY",
rcut: 5,
fcut: 1
},
AseI: {
rseq: "ATTAAT",
rcut: 4,
fcut: 2
},
AvaI: {
rseq: "CYCGRG",
rcut: 5,
fcut: 1
},
AvrII: {
rseq: "CCTAGG",
rcut: 5,
fcut: 1
},
BaeGI: {
rseq: "GKGCMC",
rcut: 1,
fcut: 5
},
BamHI: {
rseq: "GGATCC",
rcut: 5,
fcut: 1
},
BanI: {
rseq: "GGYRCC",
rcut: 5,
fcut: 1
},
BanII: {
rseq: "GRGCYC",
rcut: 1,
fcut: 5
},
BbsI: {
rseq: "GAAGACNNNNNN",
rcut: 12,
fcut: 8
},
BcgI: {
rseq: "NNNNNNNNNNNNCGANNNNNNTGCNNNNNNNNNNNN",
rcut: 34,
fcut: 36
},
BciVI: {
rseq: "GTATCCNNNNNN",
rcut: 11,
fcut: 12
},
BclI: {
rseq: "TGATCA",
rcut: 5,
fcut: 1
},
BfuAI: {
rseq: "ACCTGCNNNNNNNN",
rcut: 14,
fcut: 10
},
BglI: {
rseq: "GCCNNNNNGGC",
rcut: 4,
fcut: 7
},
BglII: {
rseq: "AGATCT",
rcut: 5,
fcut: 1
},
BlpI: {
rseq: "GCTNAGC",
rcut: 5,
fcut: 2
},
BmgBI: {
rseq: "CACGTC",
rcut: 3,
fcut: 3
},
BmrI: {
rseq: "ACTGGGNNNNN",
rcut: 10,
fcut: 11
},
BmtI: {
rseq: "GCTAGC",
rcut: 1,
fcut: 5
},
BpmI: {
rseq: "CTGGAGNNNNNNNNNNNNNNNN",
rcut: 20,
fcut: 22
},
Bpu10I: {
rseq: "CCTNAGC",
rcut: 5,
fcut: 2
},
BpuEI: {
rseq: "CTTGAGNNNNNNNNNNNNNNNN",
rcut: 20,
fcut: 22
},
BsaAI: {
rseq: "YACGTR",
rcut: 3,
fcut: 3
},
BsaBI: {
rseq: "GATNNNNATC",
rcut: 5,
fcut: 5
},
BsaHI: {
rseq: "GRCGYC",
rcut: 4,
fcut: 2
},
BsaI: {
rseq: "GGTCTCNNNNN",
rcut: 11,
fcut: 7
},
BsaWI: {
rseq: "WCCGGW",
rcut: 5,
fcut: 1
},
BsaXI: {
rseq: "NNNNNNNNNNNNACNNNNNCTCCNNNNNNNNNN",
rcut: 30,
fcut: 33
},
BseRI: {
rseq: "GAGGAGNNNNNNNNNN",
rcut: 14,
fcut: 16
},
BseYI: {
rseq: "CCCAGC",
rcut: 5,
fcut: 1
},
BsgI: {
rseq: "GTGCAGNNNNNNNNNNNNNNNN",
rcut: 20,
fcut: 22
},
BsiEI: {
rseq: "CGRYCG",
rcut: 2,
fcut: 4
},
BsiHKAI: {
rseq: "GWGCWC",
rcut: 1,
fcut: 5
},
BsiWI: {
rseq: "CGTACG",
rcut: 5,
fcut: 1
},
BsmBI: {
rseq: "CGTCTCNNNNN",
rcut: 11,
fcut: 7
},
BsmI: {
rseq: "GAATGCN",
rcut: 5,
fcut: 7
},
BsoBI: {
rseq: "CYCGRG",
rcut: 5,
fcut: 1
},
Bsp1286I: {
rseq: "GDGCHC",
rcut: 1,
fcut: 5
},
BspDI: {
rseq: "ATCGAT",
rcut: 4,
fcut: 2
},
BspEI: {
rseq: "TCCGGA",
rcut: 5,
fcut: 1
},
BspHI: {
rseq: "TCATGA",
rcut: 5,
fcut: 1
},
BspMI: {
rseq: "ACCTGCNNNNNNNN",
rcut: 14,
fcut: 10
},
BsrBI: {
rseq: "CCGCTC",
rcut: 3,
fcut: 3
},
BsrDI: {
rseq: "GCAATGNN",
rcut: 6,
fcut: 8
},
BsrFI: {
rseq: "RCCGGY",
rcut: 5,
fcut: 1
},
BsrGI: {
rseq: "TGTACA",
rcut: 5,
fcut: 1
},
BssHII: {
rseq: "GCGCGC",
rcut: 5,
fcut: 1
},
BssSI: {
rseq: "CACGAG",
rcut: 5,
fcut: 1
},
BstAPI: {
rseq: "GCANNNNNTGC",
rcut: 4,
fcut: 7
},
BstBI: {
rseq: "TTCGAA",
rcut: 4,
fcut: 2
},
BstEII: {
rseq: "GGTNACC",
rcut: 6,
fcut: 1
},
BstXI: {
rseq: "CCANNNNNNTGG",
rcut: 4,
fcut: 8
},
BstYI: {
rseq: "RGATCY",
rcut: 5,
fcut: 1
},
BstZ17I: {
rseq: "GTATAC",
rcut: 3,
fcut: 3
},
Bsu36I: {
rseq: "CCTNAGG",
rcut: 5,
fcut: 2
},
BtgI: {
rseq: "CCRYGG",
rcut: 5,
fcut: 1
},
BtgZI: {
rseq: "GCGATGNNNNNNNNNNNNNN",
rcut: 20,
fcut: 16
},
BtsI: {
rseq: "GCAGTGNN",
rcut: 6,
fcut: 8
},
ClaI: {
rseq: "ATCGAT",
rcut: 4,
fcut: 2
},
DraI: {
rseq: "TTTAAA",
rcut: 3,
fcut: 3
},
DraIII: {
rseq: "CACNNNGTG",
rcut: 3,
fcut: 6
},
DrdI: {
rseq: "GACNNNNNNGTC",
rcut: 5,
fcut: 7
},
EaeI: {
rseq: "YGGCCR",
rcut: 5,
fcut: 1
},
EagI: {
rseq: "CGGCCG",
rcut: 5,
fcut: 1
},
EarI: {
rseq: "CTCTTCNNNN",
rcut: 10,
fcut: 7
},
EciI: {
rseq: "GGCGGANNNNNNNNNNN",
rcut: 15,
fcut: 17
},
Eco53kI: {
rseq: "GAGCTC",
rcut: 3,
fcut: 3
},
EcoNI: {
rseq: "CCTNNNNNAGG",
rcut: 6,
fcut: 5
},
EcoO109I: {
rseq: "RGGNCCY",
rcut: 5,
fcut: 2
},
EcoRI: {
rseq: "GAATTC",
rcut: 5,
fcut: 1
},
EcoRV: {
rseq: "GATATC",
rcut: 3,
fcut: 3
},
Esp3I: {
rseq: "CGTCTCNNNNN",
rcut: 11,
fcut: 7
},
FspI: {
rseq: "TGCGCA",
rcut: 3,
fcut: 3
},
HaeII: {
rseq: "RGCGCY",
rcut: 1,
fcut: 5
},
HincII: {
rseq: "GTYRAC",
rcut: 3,
fcut: 3
},
HindIII: {
rseq: "AAGCTT",
rcut: 5,
fcut: 1
},
HpaI: {
rseq: "GTTAAC",
rcut: 3,
fcut: 3
},
KasI: {
rseq: "GGCGCC",
rcut: 5,
fcut: 1
},
KpnI: {
rseq: "GGTACC",
rcut: 1,
fcut: 5
},
MfeI: {
rseq: "CAATTG",
rcut: 5,
fcut: 1
},
MluI: {
rseq: "ACGCGT",
rcut: 5,
fcut: 1
},
MmeI: {
rseq: "TCCRACNNNNNNNNNNNNNNNNNNNN",
rcut: 24,
fcut: 26
},
MscI: {
rseq: "TGGCCA",
rcut: 3,
fcut: 3
},
MslI: {
rseq: "CAYNNNNRTG",
rcut: 5,
fcut: 5
},
MspA1I: {
rseq: "CMGCKG",
rcut: 3,
fcut: 3
},
NaeI: {
rseq: "GCCGGC",
rcut: 3,
fcut: 3
},
NarI: {
rseq: "GGCGCC",
rcut: 4,
fcut: 2
},
NcoI: {
rseq: "CCATGG",
rcut: 5,
fcut: 1
},
NdeI: {
rseq: "CATATG",
rcut: 4,
fcut: 2
},
NgoMIV: {
rseq: "GCCGGC",
rcut: 5,
fcut: 1
},
NheI: {
rseq: "GCTAGC",
rcut: 5,
fcut: 1
},
NmeAIII: {
rseq: "GCCGAGNNNNNNNNNNNNNNNNNNNN",
rcut: 25,
fcut: 26
},
NruI: {
rseq: "TCGCGA",
rcut: 3,
fcut: 3
},
NsiI: {
rseq: "ATGCAT",
rcut: 1,
fcut: 5
},
NspI: {
rseq: "RCATGY",
rcut: 1,
fcut: 5
},
PaeR7I: {
rseq: "CTCGAG",
rcut: 5,
fcut: 1
},
PciI: {
rseq: "ACATGT",
rcut: 5,
fcut: 1
},
PflFI: {
rseq: "GACNNNGTC",
rcut: 5,
fcut: 4
},
PflMI: {
rseq: "CCANNNNNTGG",
rcut: 4,
fcut: 7
},
PluTI: {
rseq: "GGCGCC",
rcut: 1,
fcut: 5
},
PmlI: {
rseq: "CACGTG",
rcut: 3,
fcut: 3
},
PshAI: {
rseq: "GACNNNNGTC",
rcut: 5,
fcut: 5
},
PsiI: {
rseq: "TTATAA",
rcut: 3,
fcut: 3
},
PspOMI: {
rseq: "GGGCCC",
rcut: 5,
fcut: 1
},
PstI: {
rseq: "CTGCAG",
rcut: 1,
fcut: 5
},
PvuI: {
rseq: "CGATCG",
rcut: 2,
fcut: 4
},
PvuII: {
rseq: "CAGCTG",
rcut: 3,
fcut: 3
},
SacI: {
rseq: "GAGCTC",
rcut: 1,
fcut: 5
},
SacII: {
rseq: "CCGCGG",
rcut: 2,
fcut: 4
},
SalI: {
rseq: "GTCGAC",
rcut: 5,
fcut: 1
},
ScaI: {
rseq: "AGTACT",
rcut: 3,
fcut: 3
},
SfcI: {
rseq: "CTRYAG",
rcut: 5,
fcut: 1
},
SfoI: {
rseq: "GGCGCC",
rcut: 3,
fcut: 3
},
SmaI: {
rseq: "CCCGGG",
rcut: 3,
fcut: 3
},
SmlI: {
rseq: "CTYRAG",
rcut: 5,
fcut: 1
},
SnaBI: {
rseq: "TACGTA",
rcut: 3,
fcut: 3
},
SpeI: {
rseq: "ACTAGT",
rcut: 5,
fcut: 1
},
SphI: {
rseq: "GCATGC",
rcut: 1,
fcut: 5
},
SspI: {
rseq: "AATATT",
rcut: 3,
fcut: 3
},
StuI: {
rseq: "AGGCCT",
rcut: 3,
fcut: 3
},
StyI: {
rseq: "CCWWGG",
rcut: 5,
fcut: 1
},
TspMI: {
rseq: "CCCGGG",
rcut: 5,
fcut: 1
},
Tth111I: {
rseq: "GACNNNGTC",
rcut: 5,
fcut: 4
},
XbaI: {
rseq: "TCTAGA",
rcut: 5,
fcut: 1
},
XcmI: {
rseq: "CCANNNNNNNNNTGG",
rcut: 7,
fcut: 8
},
XhoI: {
rseq: "CTCGAG",
rcut: 5,
fcut: 1
},
XmaI: {
rseq: "CCCGGG",
rcut: 5,
fcut: 1
},
XmnI: {
rseq: "GAANNNNTTC",
rcut: 5,
fcut: 5
},
ZraI: {
rseq: "GACGTC",
rcut: 3,
fcut: 3
},
AlwI: {
rseq: "GGATCNNNNN",
rcut: 10,
fcut: 9
},
ApeKI: {
rseq: "GCWGC",
rcut: 4,
fcut: 1
},
AvaII: {
rseq: "GGWCC",
rcut: 4,
fcut: 1
},
BbvI: {
rseq: "GCAGCNNNNNNNNNNNN",
rcut: 17,
fcut: 13
},
BccI: {
rseq: "CCATCNNNNN",
rcut: 10,
fcut: 9
},
BceAI: {
rseq: "ACGGCNNNNNNNNNNNNNN",
rcut: 19,
fcut: 17
},
BcoDI: {
rseq: "GTCTCNNNNN",
rcut: 10,
fcut: 6
},
BsmAI: {
rseq: "GTCTCNNNNN",
rcut: 10,
fcut: 6
},
BsmFI: {
rseq: "GGGACNNNNNNNNNNNNNN",
rcut: 19,
fcut: 15
},
BspCNI: {
rseq: "CTCAGNNNNNNNNN",
rcut: 12,
fcut: 14
},
BsrI: {
rseq: "ACTGGN",
rcut: 4,
fcut: 6
},
BstNI: {
rseq: "CCWGG",
rcut: 3,
fcut: 2
},
BtsCI: {
rseq: "GGATGNN",
rcut: 5,
fcut: 7
},
BtsIMutI: {
rseq: "CAGTGNN",
rcut: 5,
fcut: 7
},
DpnI: {
rseq: "GmATC",
rcut: 3,
fcut: 3
},
FauI: {
rseq: "CCCGCNNNNNN",
rcut: 11,
fcut: 9
},
FokI: {
rseq: "GGATGNNNNNNNNNNNNN",
rcut: 18,
fcut: 14
},
HgaI: {
rseq: "GACGCNNNNNNNNNN",
rcut: 15,
fcut: 10
},
HphI: {
rseq: "GGTGANNNNNNNN",
rcut: 12,
fcut: 13
},
Hpy99I: {
rseq: "CGWCG",
rcut: 0,
fcut: 5
},
HpyAV: {
rseq: "CCTTCNNNNNN",
rcut: 10,
fcut: 11
},
LpnPI: {
rseq: "CmCDGNNNNNNNNNNNNNN",
rcut: 19,
fcut: 15
},
MboII: {
rseq: "GAAGANNNNNNNN",
rcut: 12,
fcut: 13
},
MlyI: {
rseq: "GAGTCNNNNN",
rcut: 10,
fcut: 10
},
NciI: {
rseq: "CCSGG",
rcut: 3,
fcut: 2
},
PleI: {
rseq: "GAGTCNNNNN",
rcut: 10,
fcut: 9
},
PspGI: {
rseq: "CCWGG",
rcut: 5,
fcut: 0
},
SfaNI: {
rseq: "GCATCNNNNNNNNN",
rcut: 14,
fcut: 10
},
TfiI: {
rseq: "GAWTC",