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Localization

Christian Sieben edited this page Sep 23, 2020 · 1 revision

The ultimate starting point of SMLM data analysis is localizing molecule positions from raw microscope camera frames. Several open-source software options are available. Please find a summary in the Software section. For the description below, the molecule position list is the starting point. You can find a number of example localization files in the example_data folder.

I have uploaded some example data on the public platform Zenodo.

Introduction

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  • SMLM 101
  • [What can SMLM do for me and what not? (under constructrion)]

1. General SMLM processing

2. Photophysics, Grouping, Counting

3. Spatial Analysis

4. Tracking

5. Simulations

6. Software

7. References

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