talusR — Lightweight R toolkit for TalusBio proteomics analysis

For a full walk-through, please veiw the vignette.

Overview

talusR provides a streamlined workflow for Talus mass-spectrometry data: import, QC, differential analysis with limma, and visualization. It requires DIA-NN outputs and matching sample metadata.

Key Features

• Import & Structure
  • Load DIA-NN outputs into S4 containers: TalusDataSet or TalusDataSetList for cytoplasmic, nucleoplasmic, and chromatin-bound fractions .
  • Built on Bioconductor classes for interoperability.
• QC PCA and multivariate checks to assess clustering and detect outliers.
• Differential Analysis
  Thin wrappers around limma (empirical Baysian framework with moderated t-tests) and matrixTests (simple t-tests) for hypothesis testing.
• Visualization
  PCA, volcano, and per-protein plots powered by ggplot2.
• Bioconductor Foundations
  Uses SummarizedExperiment and S4Vectors under the hood.