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BioXAK — Biology X Analysis Kit

繁體中文說明請參閱 讀我檔案.md


Overview

BioXAK (Biology X Analysis Kit) is a free, open-source Windows desktop application that integrates multiple bioinformatics analysis workflows into a single, user-friendly platform. It is designed for wet-lab researchers who need powerful computational biology tools without requiring any programming expertise.

BioXAK is distributed as a self-contained executable requiring no programming knowledge or external runtime installation, and is freely available under the MIT License at https://github.com/bowyung/BioXAK/releases/latest.


Features

Module Description
🧬 TCGA Analysis Cancer genomics analysis using DESeq2-normalized expression data; supports differential expression, survival analysis, and multi-group comparisons with automatic statistical test selection
✂️ CRISPR sgRNA Design Automated sgRNA candidate generation with off-target scoring and GC content evaluation
💧 Flow Cytometry FCS file import, biexponential scaling, compensation matrix, and interactive gating
🔬 Primer Design Primer pair design with Tm calculation, GC content, and hairpin/dimer checking
🔎 Restriction Enzyme Analysis Restriction site mapping integrated with REBASE database
🧮 Molecular Calculators DNA/RNA/protein tools including MW calculator, codon usage, and sequence converter

Download & Installation

  1. Go to Releases
  2. Download BioXAK-v1.0.zip
  3. Extract the ZIP file to any folder
  4. Double-click BioXAK.exe to launch

Requirements: Windows 10 / 11 (64-bit). No additional installation required.


Data

TCGA preprocessed dataset is available on Zenodo: (DOI to be added)

Download and place the data file in the Data/ folder before launching the TCGA analysis module.


Citation

If you use BioXAK in your research, please cite:

(Citation will be added upon publication)


License

This project is licensed under the MIT License.