primer2comment

create executable

pyinstaller --onefiles --console primer2comment.py

required subfolder / files

primer2comment
│   README.md
│   p2c-async.py
│
└───data
│   │   test_sequence.txt
│   │
│   └───primer
│       │   primer_collection1.csv
│       │   primer_collection2.csv
│       │   ...
│   
└───final (generated)
    │   hits_primer_collection1.csv
    │   hits_primer_collection2.csv
    │   ...
    

primer-files

probe;sequence
primer_name;ATGC - Sequence 

Note: Sequence can contain regular expressions

sequence file (fasta format)

>sequence-name
ATGC-sequence