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Analytical Code

This repository contains the analytical code accompanying the manuscript: "Clarifying risk-factor associations with quantitative breast tumor features: a pooled analysis of 24 studies."


Overview

This analysis evaluates independent, dose-response associations between established breast cancer risk factors and quantitative tumor features. The study utilizes a pooled dataset of 15,731 invasive breast cancer cases from 24 international studies.

The analytical workflow performs:

  1. Case-Case Analysis: Assesses etiologic heterogeneity to link risk factors (reproductive/hormonal factors, BMI, alcohol, smoking, family history) with categorical and quantitative measures of tumor markers (ER, PR, HER2, KI67, TP53) and histological grade.
  2. Case-Control Analysis: Estimates odds ratios (ORs) for subtype-specific risk using data from 10 population-based studies.

Bayesian False Discovery Probability (BFDP) is used to account for multiple testing in case-case analyses.

Repository Structure

  • Analysis.md: The rendered analytical report (linked above).
  • assets/: Figures.
  • code/analysis.qmd: The analysis source code.
  • code/helper/: Helper functions sourced by the analytical code.

Environment

The analysis was conducted with R version 4.4.0.

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Analytical code for the pooled analysis of 24 studies investigating associations between breast cancer risk factors and quantitative tumor features.

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