Hyperpolarized (HP) 13C-MRSI enables the examination of metabolic processes that are connected to diseases [1]. For calibration and quality assessment of MRSI, 13C-labelled reference phantoms are required [2]. Here, we present a recipe and manufacturing instructions to set up stable 13C-labelled reference solutions, including a CAD design for a 3D-printed holder that fits into 50 ml Falcons filled with NaCl solution for optimal coil loading and B0-homogeneity. The phantom was experimentally tested at 11.7 T with 1H MRI, 13C spectroscopy, and MRSI via multi-gradient-echo (MGE) IDEAL signal separation [3,4].
- The CAD designs as stl files for 3D printing the NMR tube holder that fit into 50ml Falcons.
- A conference contribution (EMIM 2024) as Poster and Video, outlining the methods to build the phantom.
- This readme file.
- A MIT open source license.
[1] Wodtke, P. et al., Quo Vadis Hyperpolarized 13C MRI? Z. Für Med. Phys. (2023); [2] Larson, P. E. et al. Current Methods for Hyperpolarized [1-13C]pyruvate MRI Human Studies. (2023); [3] Reeder, S. B. et al. Multicoil Dixon chemical species separation with an iterative least-squares estimation method. Magn. Reson. Med. 51, 35–45 (2004), [4] Reeder, S. B. et al. Iterative decomposition of water and fat with echo asymmetry and least squares estimation (IDEAL): Application with fast spin-echo imaging. Magn. Reson. Med. 54, 636–644 (2005).