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v.3.3.0#43

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N-Hoffmann merged 16 commits into
mainfrom
dev
Mar 9, 2026
Merged

v.3.3.0#43
N-Hoffmann merged 16 commits into
mainfrom
dev

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@N-Hoffmann

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  • New option to use both Bambu and StringTie2 parallely and create a single extended annotation containing novel elements from both tools (--tx_discovery both).
  • Added FEELnc options to choose number of transcripts to train on (--feelnc_mRNA , --feelnc_lncRNA) (default 3,000; minimim 100).
  • All novel transcripts, regardless if they are novel isoforms of known genes or novel transcripts from novel genes, will have their biotype predicted by FEELnc. The metromap has been modified to reflect this change.

Implement the ability to use the "both" option for "tx_discovery", which will run Bambu and Stringtie2 and merge their gtfs together. The rest of the pipeline is performed on the merged file.
- Modified workflow to ensure that all novel transcripts (both known and novel genes) will go through FEELnc
- Started implementing isoform visualisation
Fix issue when only input file is used with StringTie
- Fixed problem where MSTRG genes assigned to reference genes where still present in final count matrix. These are now removed by using gtf produced by FORMAT_GFFCOMPARE instead of the raw gtf produced by STRINGTIE:MERGE.
- Publish count matrix in final output when using "both" option
@N-Hoffmann N-Hoffmann merged commit 91f5ac2 into main Mar 9, 2026
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