TIMTOM-distribution

1. Scripts description anglesTIMToTOM.py: generate relative Euler angles of TIM relative to TOM rotate001vect.m: rotate the vector [0,0,1] according to the relative Euler angles of TIM relative to TOM showLandscape.py: show the lanscape of TIM relative to TOM
2. System requirements dependencies:NEMO-TOC[1] and TOM toolbox [2] operating systems: Any platform (linux/windows/macos) was tested
3. Demo and Instructions for use First, download all the required scripts and data, then run the anglesTIMToTOM.py script. This will generate the relative Euler angles of TIM with respect to TOM, saved as allResAngleRELION.csv.
   Next, execute the rotated001vect.m script. This will produce the coordinates of the vector [0, 0, 1] after being rotated by the relative Euler angles, and save the results in rot001vector_coord.txt.
   Finally, use the showLandscape.py script to visualize the landscape of TIM relative to TOM. The generated scatter plot will be saved as scatter.png.

Jiang, W., Wagner, J., Du, W., Plitzko, J., Baumeister, W., Beck, F., and Guo, Q. (2022). A transformation clustering algorithm and its application in polyribosomes structural profiling. Nucleic Acids Res 50, 9001-9011. Nickell,S., Forster,F., Linaroudis,A., Net,W.D., Beck,F., Hegerl,R., Baumeister,W. and Plitzko,J.M. (2005) TOM software toolbox: acquisition and analysis for electron tomography. J. Struct. Biol., 149, 227–234.