diff --git a/v3/UNRELEASED_CHANGELOG.md b/v3/UNRELEASED_CHANGELOG.md
index 7fd94333899..d996434af59 100644
--- a/v3/UNRELEASED_CHANGELOG.md
+++ b/v3/UNRELEASED_CHANGELOG.md
@@ -23,6 +23,7 @@ After processing, the content will be moved to the main changelog and this file
 
 ## Fixed
 <!-- Bug fixes -->
+- Prevent macOS applications from crashing when replacing the application menu from a Wails callback
 - macOS app activation now respects activation policy for regular apps only in [PR](https://github.com/wailsapp/wails/pull/5897) by @julianstorer
 - Guard uninitialized GTK windows in Linux builds in [PR](https://github.com/wailsapp/wails/pull/5898) by @julianstorer
 - Set explicit opaque background color for Linux WebKit windows before URL load in [PR](https://github.com/wailsapp/wails/pull/5899) by @julianstorer
diff --git a/v3/pkg/application/application_darwin.go b/v3/pkg/application/application_darwin.go
index ece33619e26..b9f6f361f8b 100644
--- a/v3/pkg/application/application_darwin.go
+++ b/v3/pkg/application/application_darwin.go
@@ -141,7 +141,19 @@ static void destroyApp(void) {
 // Set the application menu
 static void setApplicationMenu(void *menu) {
 	NSMenu *nsMenu = (__bridge NSMenu *)menu;
-	[NSApp setMainMenu:menu];
+	void (^apply)(void) = ^{
+		[NSApp setMainMenu:nsMenu];
+	};
+
+	// AppKit requires the main menu to be replaced on the main thread. Menu.Set
+	// may be called from Wails event listeners, which execute on worker
+	// goroutines, so marshal the update synchronously. Avoid dispatch_sync when
+	// already on the main thread because that would deadlock.
+	if ([NSThread isMainThread]) {
+		apply();
+	} else {
+		dispatch_sync(dispatch_get_main_queue(), apply);
+	}
 }
 
 // Get the application name