MS-DIAL Crashing with Direct Infusion Data from SCIEX QTRAP API 4000

[Mudjgiwa]

I am encountering an issue while processing direct infusion lipidomics data, and I would greatly appreciate your assistance.
System and Software Details
Instrument: SCIEX QTRAP API 4000
Acquisition Method: Direct Infusion with Multi Precursor Ion Scan
Software: MS-DIAL version 5.4
Analysis Type: Lipidomics
Current Situation
I am attempting to process direct infusion data from Multi Precursor Ion Scan experiments, but the software consistently crashes during processing in MS-DIAL. This issue persists even after I separated the files into positive and negative ionization modes.
MS-DIAL processes LC-MS DDA proteomics data without issues, so the problem seems specific to the lipidomics data.
Troubleshooting Steps Taken
Initially attempted to process unseparated raw files (positive and negative ions together).
Separated lipidomics data into positive and negative mode files and tried again.
Tested MS-DIAL functionality with other data types (LC-MS DDA proteomics), which worked successfully.
Questions
Is MS-DIAL compatible with direct infusion data from Multi Precursor Ion Scan experiments?
Could the issue be related to specific data acquisition parameters rather than software compatibility?
Are there any particular settings or requirements for processing direct infusion data in MS-DIAL?
Specific Requirements
I would appreciate guidance on whether multi-precursor ions scan direct infusion data is supported in MS-DIAL.
If supported, what are the correct parameters for processing such data?
Are there any known limitations or special considerations for handling raw data from a QTRAP API 4000?