diff --git a/.changelog/4841.fixed b/.changelog/4841.fixed
new file mode 100644
index 0000000000..761e12d539
--- /dev/null
+++ b/.changelog/4841.fixed
@@ -0,0 +1 @@
+`opentelemetry-instrumentation-botocore`: Restore PyPy test coverage.
diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml
index f27399a863..58bf2a2196 100644
--- a/.github/workflows/test.yml
+++ b/.github/workflows/test.yml
@@ -3387,6 +3387,44 @@ jobs:
       - name: Run tests
         run: tox -e py314-test-instrumentation-botocore-3 -- -ra
 
+  pypy3-test-instrumentation-botocore-1-wrapt2_ubuntu-latest:
+    name: instrumentation-botocore-1-wrapt2 pypy-3.10 Ubuntu
+    runs-on: ubuntu-latest
+    timeout-minutes: 30
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python pypy-3.10
+        uses: actions/setup-python@v5
+        with:
+          python-version: "pypy-3.10"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e pypy3-test-instrumentation-botocore-1-wrapt2 -- -ra
+
+  pypy3-test-instrumentation-botocore-3_ubuntu-latest:
+    name: instrumentation-botocore-3 pypy-3.10 Ubuntu
+    runs-on: ubuntu-latest
+    timeout-minutes: 30
+    steps:
+      - name: Checkout repo @ SHA - ${{ github.sha }}
+        uses: actions/checkout@v4
+
+      - name: Set up Python pypy-3.10
+        uses: actions/setup-python@v5
+        with:
+          python-version: "pypy-3.10"
+
+      - name: Install tox
+        run: pip install tox-uv
+
+      - name: Run tests
+        run: tox -e pypy3-test-instrumentation-botocore-3 -- -ra
+
   py310-test-instrumentation-boto3sqs_ubuntu-latest:
     name: instrumentation-boto3sqs 3.10 Ubuntu
     runs-on: ubuntu-latest
diff --git a/tox.ini b/tox.ini
index 81ba52d1f5..6e0993e9e0 100644
--- a/tox.ini
+++ b/tox.ini
@@ -95,8 +95,8 @@ envlist =
     ; opentelemetry-instrumentation-botocore
     py3{10,11,12,13,14}-test-instrumentation-botocore-{0,1}-{wrapt1,wrapt2}
     py3{10,11,12,13,14}-test-instrumentation-botocore-{2,3}
-    ; FIXME: see https://github.com/open-telemetry/opentelemetry-python-contrib/issues/1736
-    ; pypy3-test-instrumentation-botocore
+    pypy3-test-instrumentation-botocore-1-wrapt2
+    pypy3-test-instrumentation-botocore-3
     lint-instrumentation-botocore
 
     ; opentelemetry-instrumentation-boto3sqs