How to fix the "tid" error when using StepMINFLUX in SMAP?

[LBR-FUYI]

Dear Ries' lab members,

I am trying to follow your great protocol (https://doi.org/10.1007/978-1-0716-4280-1_5) to track the single kinesin. When I test the StepMINFLUX in SMAP, I successfully got some data at the first time using same parameters listed in paper, but failed to do that for others and failed to repeat the original one, the error report are as below, but as I check the .mat file exported from Abberior Imspector does contain the "tid" parameters. I also attached the window when this happened. I want to know how could I solve the problem, and my MATLAB version is 2023a.

'''
Unrecognized field name "tid".

Error in ROIManager.Evaluate.StepsMINFLUX/run (line 80)
obj.index=obj.locsuse.(fid)==obj.id & obj.locsuse.filenumber ==filenumberh;

Error in interfaces.SEEvaluationProcessor/evaluate (line 83)
out=obj.run(p);

Error in gui.SEEvaluationGui>evaluatesite (line 231)
module.evaluate(site);

Error in gui.SEEvaluationGui/evaluate (line 48)
evaluatesite(obj,site);

Error in gui.SEExploreGui>plotsite (line 618)
obj.SE.processors.eval.evaluate(obj.SE.currentsite);

Error in gui.SEExploreGui>cellaxclick (line 406)
plotsite(obj,obj.SE.currentsite);

Error while evaluating Axes ButtonDownFcn.
'''

Looking for your reply, thank you!

[Rieslab]

Hello,Thanks for letting me know and sorry for the bug. I am currently traveling, but will get to it next week. Can you try both the develop and the master branch to see if one of them works? Are you talking about the data files that are part of the publication?Thanks,Jonas On 14. May 2025, at 15:56, BINRUN ***@***.***> wrote: LBR-FUYI created an issue (jries/SMAP#29) Dear Ries' lab members, I am trying to follow your great protocol (https://doi.org/10.1007/978-1-0716-4280-1_5) to track the single kinesin. When I test the StepMINFLUX in SMAP, I successfully got some data at the first time using same parameters listed in paper, but failed to do that for others and failed to repeat the original one, the error report are as below, but as I check the .mat file exported from Abberior Imspector does contain the "tid" parameters. I also attached the window when this happened. I want to know how could I solve the problem, and my MATLAB version is 2023a. ''' Unrecognized field name "tid". Error in ROIManager.Evaluate.StepsMINFLUX/run (line 80) obj.index=obj.locsuse.(fid)==obj.id & obj.locsuse.filenumber ==filenumberh; Error in interfaces.SEEvaluationProcessor/evaluate (line 83) out=obj.run(p); Error in gui.SEEvaluationGui>evaluatesite (line 231) module.evaluate(site); Error in gui.SEEvaluationGui/evaluate (line 48) evaluatesite(obj,site); Error in gui.SEExploreGui>plotsite (line 618) obj.SE.processors.eval.evaluate(obj.SE.currentsite); Error in gui.SEExploreGui>cellaxclick (line 406) plotsite(obj,obj.SE.currentsite); Error while evaluating Axes ButtonDownFcn. ''' image.png (view on web) Looking for your reply, thank you! —Reply to this email directly, view it on GitHub, or unsubscribe.You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>

[LBR-FUYI]

Dear Jonas,

Thanks for your reply! I have tried the master branch of the SMAP, and my test data is generated by my own experiment using Abberior MINFLUX and outputed as .mat by Abberior Imspector, we would like to repeat the great kinesin trajectories you showed in publication. I also tested the data provided with your publication, they all went well, but for my own data I found that when I click the "Load only Main Field" when importing data using "Abbrior MINFLUX", the following stepfitting function will raise error as I mentioned, if not clicked it could run normally. As I used the data you kindly provide with the publication, it all went well. Could I know what the "Main Field" indicate exactly and how will it affect the fitting?

Also, I tried the "develop" branch as there are many branches, but it results in failure of loaing data and returns errors as below:
'''
Unrecognized field name "simple".

Error in File.Load.Loader_minflux_json>loadfile (line 65)
loc=minfluxmat2loc(jt,p.onlyvalid,~p.simple);

Error in File.Load.Loader_minflux_json/load (line 12)
loadfile(obj,p,file);

Error in gui.GuiFile>loadfiles (line 339)
loader.load(par,[pfad f]);

Error in gui.GuiFile/loadbutton_callback (line 145)
loadfiles(obj,loader,f{k},pfad)

Error in gui.GuiFile>@(varargin)obj.loadbutton_callback(varargin{:}) (line 414)
pard.load.object=struct('Style','pushbutton','String','Load','FontWeight','bold','Callback',{{@obj.loadbutton_callback,0}});

Error while evaluating UIControl Callback.
'''

Hope you enjoy your trip and look forward to your reply!

[Rieslab]

Hi,The problem is that with every new update, Imspector changes the file format slightly, and for me it is not easy to stay on top of this. You are welcome to send me one of your files, then I will try to modify the loader.Load only main field only loads the most important properties of a localization, but not additional ones that we mostly use for quality control. You could go ahead with those.Best,JonasOn 17. May 2025, at 09:29, BINRUN ***@***.***> wrote: LBR-FUYI left a comment (jries/SMAP#29) Dear Jonas, Thanks for your reply! I have tried the master branch of the SMAP, and my test data is generated by my own experiment using Abberior MINFLUX and outputed as .mat by Abberior Imspector, we would like to repeat the great kinesin trajectories you showed in publication. I also tested the data provided with your publication, they all went well, but for my own data I found that when I click the "Load only Main Field" when importing data using "Abbrior MINFLUX", the following stepfitting function will raise error as I mentioned, if not clicked it could run normally. As I used the data you kindly provide with the publication, it all went well. Could I know what the "Main Field" indicate exactly and how will it affect the fitting? image.png (view on web) Also, I tried the "develop" branch as there are many branches, but it results in failure of loaing data and returns errors as below: ''' Unrecognized field name "simple". Error in File.Load.Loader_minflux_json>loadfile (line 65) loc=minfluxmat2loc(jt,p.onlyvalid,~p.simple); Error in File.Load.Loader_minflux_json/load (line 12) loadfile(obj,p,file); Error in gui.GuiFile>loadfiles (line 339) loader.load(par,[pfad f]); Error in gui.GuiFile/loadbutton_callback (line 145) loadfiles(obj,loader,f{k},pfad) Error in gui.GuiFile>@(varargin)obj.loadbutton_callback(varargin{:}) (line 414) ***@***.***_callback,0}}); Error while evaluating UIControl Callback. ''' Hope you enjoy your trip and look forward to your reply! —Reply to this email directly, view it on GitHub, or unsubscribe.You are receiving this because you commented.Message ID: ***@***.***>