Hi there,
Thanks so much for building and open-sourcing SenePy! It's an amazing tool that really solves the big problem of cell-type-specific senescence heterogeneity, and it performs way better than traditional markers like p16/p21 for in vivo data.
We're currently working on mouse testicular aging using single-cell RNA-seq, comparing senescence burden across different experimental groups. After evaluating all available tools, we think SenePy is the best fit for our study.
Our only concern is that there are no pre-built senescence signatures for mouse testis cell types (Sertoli cells, Leydig cells, spermatogonia, etc.). We're worried the universal mouse signature might not be optimal here because:
Testis has highly proliferative germ cells with unique transcriptional profiles
Testicular somatic cells have specialized functions and distinct senescence mechanisms
Germ cells maintain high telomerase activity, unlike most somatic cells
We'd really appreciate your advice on:
Do you have any unpublished testis signatures you could share?
What's your recommended way to build custom testis signatures using the SenePy framework? Should we follow your paper's exact method with
Tabula Muris Senis testis data?
Are there any plans to add testis support in future updates?
We'd be happy to contribute our own testis scRNA-seq data to help improve the tool if that's useful.
Thanks again for your great work!
Hi there,
Thanks so much for building and open-sourcing SenePy! It's an amazing tool that really solves the big problem of cell-type-specific senescence heterogeneity, and it performs way better than traditional markers like p16/p21 for in vivo data.
We're currently working on mouse testicular aging using single-cell RNA-seq, comparing senescence burden across different experimental groups. After evaluating all available tools, we think SenePy is the best fit for our study.
Our only concern is that there are no pre-built senescence signatures for mouse testis cell types (Sertoli cells, Leydig cells, spermatogonia, etc.). We're worried the universal mouse signature might not be optimal here because:
Testis has highly proliferative germ cells with unique transcriptional profiles
Testicular somatic cells have specialized functions and distinct senescence mechanisms
Germ cells maintain high telomerase activity, unlike most somatic cells
We'd really appreciate your advice on:
Do you have any unpublished testis signatures you could share?
What's your recommended way to build custom testis signatures using the SenePy framework? Should we follow your paper's exact method with
Tabula Muris Senis testis data?
Are there any plans to add testis support in future updates?
We'd be happy to contribute our own testis scRNA-seq data to help improve the tool if that's useful.
Thanks again for your great work!