Original report by Jason Vander Heiden (Bitbucket: javh, GitHub: javh).
We could use either a small example or a full workflow for data where the UMI is after the primer region (using MaskPrimers-extract).
Example protocol:
Turchaninova, M. A. et al. High-quality full-length immunoglobulin profiling with unique molecular barcoding. Nat. Protoc. 11, 1599–1616 (2016).
Though, that might just be a case of using the TS site as the primer: CTTGGGG
Original report by Jason Vander Heiden (Bitbucket: javh, GitHub: javh).
We could use either a small example or a full workflow for data where the UMI is after the primer region (using MaskPrimers-extract).
Example protocol:
Turchaninova, M. A. et al. High-quality full-length immunoglobulin profiling with unique molecular barcoding. Nat. Protoc. 11, 1599–1616 (2016).
Though, that might just be a case of using the TS site as the primer:
CTTGGGG