Background
I am a senior sequencing engineer at Cincinnati Children's Hospital Medical Center. We are currently working with several transgenic mouse models involving CRISPR-mediated editing. We have generated Oxford Nanopore long-read data and successfully identified several insertion sites that we are looking to characterize further.
Proposal
I recently read your preprint, "ISdetector: precise mapping of insertion sequences and associated structural variations from short-read sequencing data," and noticed that long-read integration is listed as a future direction for the tool.
I am interested in validating ISdetector on our long-read datasets. Our data could serve as a practical benchmark for the tool’s expansion into long-read structural variant (SV) and insertion sequence (IS) detection, particularly in complex eukaryotic genomes like the mouse.
Contact for Collaboration
I would love to discuss a potential collaboration or act as an early tester when you begin implementing the long-read feature.
Please reach out to me at: ammar.husami at cchmc.org
Congratulations on the release of the tool!
Best regards,
Ammar
Background
I am a senior sequencing engineer at Cincinnati Children's Hospital Medical Center. We are currently working with several transgenic mouse models involving CRISPR-mediated editing. We have generated Oxford Nanopore long-read data and successfully identified several insertion sites that we are looking to characterize further.
Proposal
I recently read your preprint, "ISdetector: precise mapping of insertion sequences and associated structural variations from short-read sequencing data," and noticed that long-read integration is listed as a future direction for the tool.
I am interested in validating ISdetector on our long-read datasets. Our data could serve as a practical benchmark for the tool’s expansion into long-read structural variant (SV) and insertion sequence (IS) detection, particularly in complex eukaryotic genomes like the mouse.
Contact for Collaboration
I would love to discuss a potential collaboration or act as an early tester when you begin implementing the long-read feature.
Please reach out to me at: ammar.husami at cchmc.org
Congratulations on the release of the tool!
Best regards,
Ammar